Evaluation of cold atmospheric pressure plasma irradiation of water as a method of singlet oxygen generation

We used cold atmospheric pressure plasma jet to examine in detail 1O2 generation water. ESR with 2,2,5,5-tetramethyl-3-pyrroline-3-carboxamide, a secondary amine probe, was for the detection of 1O2. Nitroxide radical formation detected after irradiation 2,2,5,5-tetramethyl-3-pyrroline-3-carboxamide solution. An scavenger/quencher inhibited signal intensity induced by irradiation, but this inhibition not 100%. As reacts oxidizing species other than 1O2, it assumed that NaN3 corresponds only nitroxide generated The concentration produced 60 ‍s estimated at 8 ‍μM. When compared methods like rose bengal photoirradiation and 4-methyl-1,4-etheno-2,3-benzodioxin-1(4H)-propanoic acid (endoperoxide) thermal decomposition, found be, decreasing order, ≥ > endoperoxide decomposition. Cold is presumed specifically generate can be mimic states oxidative stress involving multiple ROS.